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Dextran gel
The full name is hydroxypropyl glucan gel. Sephadex lh-20 is the product of Sephadex g-25 (one of the commercial models of dextran gel according to crosslinking degree) after hydroxypropyl treatment. At this time, the glucose part of dextran gel molecule will combine with hydroxypropyl into the following ether bond form: -oh → -och2ch2ch2oh. Compared with Sephadex G, the total number of -oh groups in Sephadex lh-20 molecules did not change, but the proportion of carbon atoms was relatively increased. Therefore, Sephadex lh-20 can be used not only in water, but also in polar organic solvents or mixed solvents composed of them and water.
Dextran gel
GE Dextran gel(LH-20)
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Sephadex LH-20葡聚糖凝膠
Sephadex葡聚糖凝膠是通過表氯醇交聯(lián)多個葡聚糖得到的。Sephadex G-10、G-25這些葡聚糖凝膠是在水溶液中使用,若要用有機(jī)溶劑作為流動相,只有通過羥丙基化將Sephadex G轉(zhuǎn)化為可在有機(jī)溶劑中溶漲的葡聚糖凝膠。這時的產(chǎn)品為Sephardex LH-20。
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